The CNAG Biorepository collects, quantifies and stores the DNA and RNA samples for sequencing requests.
We work closely with our collaborators to ensure that appropriate DNA/RNA extraction procedures are followed, to determine the sample requirements for the experiments to be conducted by the CNAG and to coordinate the sample shipment. Barcode labels and tubes are sent to the collaborators for safe tracking of samples.
At the CNAG, samples are checked for purity and integrity before processing. DNA integrity is assessed on agarose gel. The DNA QC includes detection of incidental present inhibitors by regular PCR amplification. Concentration is verified by fluorometry (Picogreen assay). RNA is subjected to rigorous quality control to monitor degradation on the Agilent 2100 Bioanalyzer and quantitated by fluorometry.
Samples are stored under highly controlled conditions and, if appropriate, distributed in several aliquots to avoid thawing and refreezing.
The CNAG offers researchers access to state-of-the-art methods for high throughput sequencing for a wide range of applications.
We currently operate 1 Illumina HiSeq 4000, 2 Illumina HiSeq 2500, 3 Illumina HiSeq 2000 and 1 Illumina MiSeq.
The members of the sequencing platform are postdoctoral research scientists, engineers, and technicians who are professionally trained and form a highly qualified team to accomplish the specialized scientific tasks of sequencing. They have established, and continue to develop, many techniques to analyze the information encrypted in genomes. The platform consists of four teams:
The sequencing operation is supported by an extensive informatics infrastructure.
7.6 petabyte of data storage, 3472 of computing cores, an internal 56 Gb/s network and multiple 10 Gb/s direct physical connections to the Barcelona Supercomputing Center which has over 48.000 compute cores.
Several bioinformatic teams develop methods for different kinds of applications and support the analysis of large-scale sequencing data:
- Production Bioinformatics
- Data Analysis
- Genome Assembly and Annotation
- Bionformatics Development and Statistical Genomics
- Structural Genomics
- Comparative Genomics
- Biomedical Genomics
- Population Genomics
- Functional Genomics